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3 plasmids (pGL1124, pGL1224 and pGL1217) have been created to enable the substitution of one allele of CYC9Lister 427 cells. Trypanosomes have been transfected with Each individual plasmid independently, then subjected to a next transfection with among the list of other plasmids, with a unique resistance gene, with all pairwise mixtures carried ou